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Experimental and Computational Mutagenesis To Investigate the Positioning of a General Base within an Enzyme Active Site
[Image: see text] The positioning of catalytic groups within proteins plays an important role in enzyme catalysis, and here we investigate the positioning of the general base in the enzyme ketosteroid isomerase (KSI). The oxygen atoms of Asp38, the general base in KSI, were previously shown to be in...
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| Main Authors: | , , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American
Chemical Society
2014
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4004248/ https://ncbi.nlm.nih.gov/pubmed/24597914 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi401671t |
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