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Application of phasor plot and autofluorescence correction for study of heterogeneous cell population

Protein-protein interactions in cells are often studied using fluorescence resonance energy transfer (FRET) phenomenon by fluorescence lifetime imaging microscopy (FLIM). Here, we demonstrate approaches to the quantitative analysis of FRET in cell population in a case complicated by a highly heterog...

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Bibliografske podrobnosti
Main Authors: Szmacinski, Henryk, Toshchakov, Vladimir, Lakowicz, Joseph R.
Format: Artigo
Jezik:Inglês
Izdano: Society of Photo-Optical Instrumentation Engineers 2014
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC4000004/
https://ncbi.nlm.nih.gov/pubmed/24770662
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1117/1.JBO.19.4.046017
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