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Application of phasor plot and autofluorescence correction for study of heterogeneous cell population

Protein-protein interactions in cells are often studied using fluorescence resonance energy transfer (FRET) phenomenon by fluorescence lifetime imaging microscopy (FLIM). Here, we demonstrate approaches to the quantitative analysis of FRET in cell population in a case complicated by a highly heterog...

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Detalhes bibliográficos
Main Authors: Szmacinski, Henryk, Toshchakov, Vladimir, Lakowicz, Joseph R.
Formato: Artigo
Idioma:Inglês
Publicado em: Society of Photo-Optical Instrumentation Engineers 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4000004/
https://ncbi.nlm.nih.gov/pubmed/24770662
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1117/1.JBO.19.4.046017
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