A simple and accurate HPLC method for fecal bile acid profile in healthy and cirrhotic subjects: validation by GC-MS and LC-MS

We have developed a simple and accurate HPLC method for measurement of fecal bile acids using phenacyl derivatives of unconjugated bile acids, and applied it to the measurement of fecal bile acids in cirrhotic patients. The HPLC method has the following steps: 1) lyophilization of the stool sample;...

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Detalhes bibliográficos
Main Authors: Kakiyama, Genta, Muto, Akina, Takei, Hajime, Nittono, Hiroshi, Murai, Tsuyoshi, Kurosawa, Takao, Hofmann, Alan F., Pandak, William M., Bajaj, Jasmohan S.
Formato: Artigo
Idioma:Inglês
Publicado em: The American Society for Biochemistry and Molecular Biology 2014
Assuntos:
Methods
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3995475/
https://ncbi.nlm.nih.gov/pubmed/24627129
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1194/jlr.D047506
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