A simple and accurate HPLC method for fecal bile acid profile in healthy and cirrhotic subjects: validation by GC-MS and LC-MS

We have developed a simple and accurate HPLC method for measurement of fecal bile acids using phenacyl derivatives of unconjugated bile acids, and applied it to the measurement of fecal bile acids in cirrhotic patients. The HPLC method has the following steps: 1) lyophilization of the stool sample;...

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Dettagli Bibliografici
Autori principali: Kakiyama, Genta, Muto, Akina, Takei, Hajime, Nittono, Hiroshi, Murai, Tsuyoshi, Kurosawa, Takao, Hofmann, Alan F., Pandak, William M., Bajaj, Jasmohan S.
Natura: Artigo
Lingua:Inglês
Pubblicazione: The American Society for Biochemistry and Molecular Biology 2014
Soggetti:
Methods
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3995475/
https://ncbi.nlm.nih.gov/pubmed/24627129
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1194/jlr.D047506
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