Top-Down Proteomic Identification of Shiga Toxin 2 Subtypes from Shiga Toxin-Producing Escherichia coli by Matrix-Assisted Laser Desorption Ionization–Tandem Time of Flight Mass Spectrometry

We have analyzed 26 Shiga toxin-producing Escherichia coli (STEC) strains for Shiga toxin 2 (Stx2) production using matrix-assisted laser desorption ionization (MALDI)–tandem time of flight (TOF-TOF) tandem mass spectrometry (MS/MS) and top-down proteomic analysis. STEC strains were induced to overe...

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Bibliographic Details
Main Authors: Fagerquist, Clifton K., Zaragoza, William J., Sultan, Omar, Woo, Nathan, Quiñones, Beatriz, Cooley, Michael B., Mandrell, Robert E.
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 2014
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Methods
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3993300/
https://ncbi.nlm.nih.gov/pubmed/24584253
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.04058-13
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