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Top-down and middle-down proteomic analysis of Shiga toxin using MALDI-TOF-TOF mass spectrometry
The method describes a step-by-step process for analysis of putative Shiga toxin-producing Escherichia coli (STEC) for expression of Shiga toxin (Stx). The technique utilizes antibiotic induction, mass spectrometry and top-down/middle-down proteomic analysis. Stx expression is induced by overnight c...
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| Pubblicato in: | MethodsX |
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| Autori principali: | , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Elsevier
2019
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6484209/ https://ncbi.nlm.nih.gov/pubmed/31049298 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.mex.2019.04.011 |
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