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Gene and Protein Sequence Optimization for High-Level Production of Fully Active and Aglycosylated Lysostaphin in Pichia pastoris

Lysostaphin represents a promising therapeutic agent for the treatment of staphylococcal infections, in particular those of methicillin-resistant Staphylococcus aureus (MRSA). However, conventional expression systems for the enzyme suffer from various limitations, and there remains a need for an eff...

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Detalhes bibliográficos
Main Authors: Zhao, Hongliang, Blazanovic, Kristina, Choi, Yoonjoo, Bailey-Kellogg, Chris, Griswold, Karl E.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3993296/
https://ncbi.nlm.nih.gov/pubmed/24561590
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.03914-13
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