Thermostability Improvement of a Streptomyces Xylanase by Introducing Proline and Glutamic Acid Residues

Protein engineering is commonly used to improve the robustness of enzymes for activity and stability at high temperatures. In this study, we identified four residues expected to affect the thermostability of Streptomyces sp. strain S9 xylanase XynAS9 through multiple-sequence analysis (MSA) and mole...

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Detalhes bibliográficos
Main Authors: Wang, Kun, Luo, Huiying, Tian, Jian, Turunen, Ossi, Huang, Huoqing, Shi, Pengjun, Hua, Huifang, Wang, Caihong, Wang, Shuanghe, Yao, Bin
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2014
Assuntos:
Enzymology and Protein Engineering
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3993148/
https://ncbi.nlm.nih.gov/pubmed/24463976
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.03458-13
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