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High-affinity gold nanoparticle pin to label and localize histidine-tagged protein in macromolecular assemblies

There is significant demand for experimental approaches to aid protein localization in electron microscopy micrographs and ultimately in three-dimensional reconstructions of macromolecular assemblies. We report preparation and use of a reagent consisting of tris-nitrilotriacetic acid (tris-NTA) conj...

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Hlavní autoři: Anthony, Kelsey C., You, Changjiang, Piehler, Jacob, Pomeranz Krummel, Daniel A.
Médium: Artigo
Jazyk:Inglês
Vydáno: 2014
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3984384/
https://ncbi.nlm.nih.gov/pubmed/24560806
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.str.2014.01.007
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