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High-affinity gold nanoparticle pin to label and localize histidine-tagged protein in macromolecular assemblies
There is significant demand for experimental approaches to aid protein localization in electron microscopy micrographs and ultimately in three-dimensional reconstructions of macromolecular assemblies. We report preparation and use of a reagent consisting of tris-nitrilotriacetic acid (tris-NTA) conj...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3984384/ https://ncbi.nlm.nih.gov/pubmed/24560806 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.str.2014.01.007 |
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