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Nuclear Patch-Clamp Electrophysiology of Ca(2+) Channels

Patch-clamping the outer or inner nuclear membrane of isolated nuclei is very similar to patch-clamping the plasma membrane of isolated cells. This protocol describes in detail all the steps required to successfully obtain nuclear membrane patches, in various configurations, from both the outer and...

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Bibliografske podrobnosti
Main Authors: Mak, Don-On Daniel, Vais, Horia, Cheung, King-Ho, Foskett, J. Kevin
Format: Artigo
Jezik:Inglês
Izdano: 2013
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC3984033/
https://ncbi.nlm.nih.gov/pubmed/24003194
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/pdb.prot073064
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