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Nuclear Patch-Clamp Electrophysiology of Ca(2+) Channels

Patch-clamping the outer or inner nuclear membrane of isolated nuclei is very similar to patch-clamping the plasma membrane of isolated cells. This protocol describes in detail all the steps required to successfully obtain nuclear membrane patches, in various configurations, from both the outer and...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Prif Awduron: Mak, Don-On Daniel, Vais, Horia, Cheung, King-Ho, Foskett, J. Kevin
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: 2013
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC3984033/
https://ncbi.nlm.nih.gov/pubmed/24003194
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/pdb.prot073064
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