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Nuclear Patch-Clamp Electrophysiology of Ca(2+) Channels
Patch-clamping the outer or inner nuclear membrane of isolated nuclei is very similar to patch-clamping the plasma membrane of isolated cells. This protocol describes in detail all the steps required to successfully obtain nuclear membrane patches, in various configurations, from both the outer and...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3984033/ https://ncbi.nlm.nih.gov/pubmed/24003194 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/pdb.prot073064 |
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