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A 5000-Fold Increase in the Specificity of a Bacterial Phosphotriesterase for Malathion through Combinatorial Active Site Mutagenesis

Phosphotriesterases (PTEs) have been isolated from a range of bacterial species, including Agrobcaterium radiobacter (PTE(Ar)), and are efficient enzymes with broad substrate ranges. The turnover rate of PTE(Ar) for the common organophosphorous insecticide malathion is lower than expected based on i...

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Hlavní autoři: Naqvi, Tatheer, Warden, Andrew C., French, Nigel, Sugrue, Elena, Carr, Paul D., Jackson, Colin J., Scott, Colin
Médium: Artigo
Jazyk:Inglês
Vydáno: Public Library of Science 2014
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3983157/
https://ncbi.nlm.nih.gov/pubmed/24721933
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0094177
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