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Anomalous scattering analysis of Agrobacterium radiobacter phosphotriesterase: the prominent role of iron in the heterobinuclear active site

Bacterial phosphotriesterases are binuclear metalloproteins for which the catalytic mechanism has been studied with a variety of techniques, principally using active sites reconstituted in vitro from apoenzymes. Here, atomic absorption spectroscopy and anomalous X-ray scattering have been used to de...

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Detalhes bibliográficos
Main Authors: Jackson, Colin J., Carr, Paul D., Kim, Hye-Kyung, Liu, Jian-Wei, Herrald, Paul, Mitić, Nataša, Schenk, Gerhard, Smith, Clyde A., Ollis, David L.
Formato: Artigo
Idioma:Inglês
Publicado em: Portland Press Ltd. 2006
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1533316/
https://ncbi.nlm.nih.gov/pubmed/16686603
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1042/BJ20060276
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