Efficient trans-cleavage of a stem-loop RNA substrate by a ribozyme derived from neurospora VS RNA.
We have constructed a ribozyme containing 144 nucleotides of Neurospora VS RNA that can catalyze the cleavage of a separate RNA in a true enzymatic manner (Km approximately 0.13 microM, kcat approximately 0.7/min). Comparison of the rates of cis- and trans-cleavage, as well as the lack of effect of...
Shranjeno v:
| izdano v: | EMBO J |
|---|---|
| Principais autores: | , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
Nature Publishing Group
1995
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC398091/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7835347/ https://ncbi.nlm.nih.govhttps://doi.org/10.1002/j.1460-2075.1995.tb07011.x |
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