Efficient trans-cleavage of a stem-loop RNA substrate by a ribozyme derived from neurospora VS RNA.

We have constructed a ribozyme containing 144 nucleotides of Neurospora VS RNA that can catalyze the cleavage of a separate RNA in a true enzymatic manner (Km approximately 0.13 microM, kcat approximately 0.7/min). Comparison of the rates of cis- and trans-cleavage, as well as the lack of effect of...

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Pubblicato in:	EMBO J
Autori principali:	Guo, H C, Collins, R A
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	Nature Publishing Group 1995
Soggetti:	Research Article
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC398091/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7835347/ https://ncbi.nlm.nih.govhttps://doi.org/10.1002/j.1460-2075.1995.tb07011.x
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