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Two-codon insertion mutagenesis of plasmid genes by using single-stranded hexameric oligonucleotides.
An efficient method for introducing two codons into a cloned gene has been applied to studying functional regions of the pBR322-encoded tetracycline-resistance gene and beta-lactamase (ampicillin-resistance) gene. Single-stranded hexameric linkers are inserted into a preexisting cohesive end restric...
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
1985
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| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC397964/ https://ncbi.nlm.nih.gov/pubmed/3889924 |
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