Two-codon insertion mutagenesis of plasmid genes by using single-stranded hexameric oligonucleotides.
An efficient method for introducing two codons into a cloned gene has been applied to studying functional regions of the pBR322-encoded tetracycline-resistance gene and beta-lactamase (ampicillin-resistance) gene. Single-stranded hexameric linkers are inserted into a preexisting cohesive end restric...
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| Pubblicato in: | Proc Natl Acad Sci U S A |
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| Autore principale: | |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
National Academy of Sciences
1985
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC397964/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3889924/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.82.12.4202 |
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