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Isolating Nuclei from Cultured Cells for Patch-Clamp Electrophysiology of Intracellular Ca(2+) Channels
Nuclear patch-clamp experiments can be performed with intact nuclei or with nuclei from which the outer nuclear membrane has been removed. This protocol presents procedures for harvesting different types of cultured cells, isolating nuclei, and exposing the inner nuclear membrane by agitating in the...
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| 主要な著者: | , , , |
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| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
2013
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3979423/ https://ncbi.nlm.nih.gov/pubmed/24003193 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/pdb.prot073056 |
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