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Negative selection and stringency modulation in phage-assisted constinuous evolution
Phage-assisted continuous evolution (PACE) uses a modified filamentous bacteriophage life cycle to dramatically accelerate laboratory evolution experiments. In this work we expand the scope and capabilities of the PACE method with two key advances that enable the evolution of biomolecules with radic...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3977541/ https://ncbi.nlm.nih.gov/pubmed/24487694 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nchembio.1453 |
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