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Development of a multiplex PCR assay to detect Edwardsiella tarda, Streptococcus parauberis, and Streptococcus iniae in olive flounder (Paralichthys olivaceus)

A multiplex PCR protocol was established to simultaneously detect major bacterial pathogens in olive flounder (Paralichthys olivaceus) including Edwardsiella (E.) tarda, Streptococcus (S.) parauberis, and S. iniae. The PCR assay was able to detect 0.01 ng of E. tarda, 0.1 ng of S. parauberis, and 1...

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Main Authors: Park, Seong Bin, Kwon, Kyoung, Cha, In Seok, Jang, Ho Bin, Nho, Seong Won, Fagutao, Fernand F., Kim, Young Kyu, Yu, Jong Earn, Jung, Tae Sung
格式: Artigo
語言:Inglês
出版: The Korean Society of Veterinary Science 2014
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC3973760/
https://ncbi.nlm.nih.gov/pubmed/24378589
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4142/jvs.2014.15.1.163
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