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Development of a multiplex PCR assay to detect Edwardsiella tarda, Streptococcus parauberis, and Streptococcus iniae in olive flounder (Paralichthys olivaceus)

A multiplex PCR protocol was established to simultaneously detect major bacterial pathogens in olive flounder (Paralichthys olivaceus) including Edwardsiella (E.) tarda, Streptococcus (S.) parauberis, and S. iniae. The PCR assay was able to detect 0.01 ng of E. tarda, 0.1 ng of S. parauberis, and 1...

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Hlavní autoři: Park, Seong Bin, Kwon, Kyoung, Cha, In Seok, Jang, Ho Bin, Nho, Seong Won, Fagutao, Fernand F., Kim, Young Kyu, Yu, Jong Earn, Jung, Tae Sung
Médium: Artigo
Jazyk:Inglês
Vydáno: The Korean Society of Veterinary Science 2014
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3973760/
https://ncbi.nlm.nih.gov/pubmed/24378589
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4142/jvs.2014.15.1.163
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