Development of a multiplex PCR assay to detect Edwardsiella tarda, Streptococcus parauberis, and Streptococcus iniae in olive flounder (Paralichthys olivaceus)

A multiplex PCR protocol was established to simultaneously detect major bacterial pathogens in olive flounder (Paralichthys olivaceus) including Edwardsiella (E.) tarda, Streptococcus (S.) parauberis, and S. iniae. The PCR assay was able to detect 0.01 ng of E. tarda, 0.1 ng of S. parauberis, and 1...

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Detalhes bibliográficos
Main Authors: Park, Seong Bin, Kwon, Kyoung, Cha, In Seok, Jang, Ho Bin, Nho, Seong Won, Fagutao, Fernand F., Kim, Young Kyu, Yu, Jong Earn, Jung, Tae Sung
Formato: Artigo
Idioma:Inglês
Publicado em: The Korean Society of Veterinary Science 2014
Assuntos:
Short Communication
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3973760/
https://ncbi.nlm.nih.gov/pubmed/24378589
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4142/jvs.2014.15.1.163
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