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Efficient cloning of single-copy genes using specialized cosmid vectors: isolation of mutant dihydrofolate reductase genes.

A method for the efficient cloning of single-copy genes from restriction digests of mammalian DNA is described. The method is illustrated by the cloning of several mutant genes as well as the wild-type gene for Chinese hamster dihydrofolate reductase (DHFR; 7,8-dihydrofolate:NADP+ oxidoreductase, EC...

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Bibliografiske detaljer
Main Authors: Urlaub, G, Carothers, A M, Chasin, L A
Format: Artigo
Sprog:Inglês
Udgivet: 1985
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC397220/
https://ncbi.nlm.nih.gov/pubmed/3883353
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