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Determination of the stereostructure of the product of Tn3 resolvase by a general method.

A method has been developed for determination of the absolute structure of DNA catenanes. The catenated DNA is partially denatured before being thickened with a coating of RecA protein and spread for electron microscopy. This treatment allows visualization of the orientation of each ring as well as...

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Bibliografiske detaljer
Udgivet i:Proc Natl Acad Sci U S A
Main Authors: Wasserman, S A, Cozzarelli, N R
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1985
Fag:
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC397197/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2983329/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.82.4.1079
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