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Determination of the stereostructure of the product of Tn3 resolvase by a general method.

A method has been developed for determination of the absolute structure of DNA catenanes. The catenated DNA is partially denatured before being thickened with a coating of RecA protein and spread for electron microscopy. This treatment allows visualization of the orientation of each ring as well as...

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Autors principals: Wasserman, S A, Cozzarelli, N R
Format: Artigo
Idioma:Inglês
Publicat: 1985
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC397197/
https://ncbi.nlm.nih.gov/pubmed/2983329
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