Isolation and characterization of the Tn3 resolvase synaptic intermediate.

We have isolated in quantitative yield the synaptic intermediate formed during site-specific recombination by Tn3 resolvase and characterized it by restriction endonuclease mapping, electron microscopy and topological methods. The intermediate accumulates at low reaction temperatures and is stabiliz...

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Bibliographic Details
Published in:EMBO J
Main Authors: Benjamin, H W, Cozzarelli, N R
Format: Artigo
Language:Inglês
Published: Nature Publishing Group 1988
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC457183/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2844528/
https://ncbi.nlm.nih.govhttps://doi.org/10.1002/j.1460-2075.1988.tb03023.x
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