A genetic screen for mutations that increase the thermal stability of phage T4 lysozyme.
A method has been developed to screen for mutants of phage T4 lysozyme that are more stable than the wild-type enzyme. Using an assay that detects lysozyme activity on Petri plates [Streisinger, G., Okada, Y., Emrich, J., Newton, J., Tsugita, A., Terzaghi, E. & Inouye, M. (1966) Cold Spring Harbor S...
Gespeichert in:
| Veröffentlicht in: | Proc Natl Acad Sci U S A |
|---|---|
| Hauptverfasser: | , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
National Academy of Sciences
1985
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| Schlagworte: | |
| Online-Zugang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC397123/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3856227/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.82.3.747 |
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