Loading...
In vitro expansion and genetic modification of gastrointestinal stem cells as organoids
A key issue for sustainable culture of adult epithelial cells is enrichment for stem cell populations in tissue organoids. Gastrointestinal stem cells can be propagated using conditioned media from a supportive cell line (L-WRN). This protocol describes how to prepare conditioned media and culture s...
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Artigo |
| Language: | Inglês |
| Published: |
2013
|
| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3969856/ https://ncbi.nlm.nih.gov/pubmed/24232249 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2013.153 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|