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In vitro expansion and genetic modification of gastrointestinal stem cells as organoids

A key issue for sustainable culture of adult epithelial cells is enrichment for stem cell populations in tissue organoids. Gastrointestinal stem cells can be propagated using conditioned media from a supportive cell line (L-WRN). This protocol describes how to prepare conditioned media and culture s...

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Detalhes bibliográficos
Main Authors: Miyoshi, Hiroyuki, Stappenbeck, Thaddeus S.
Formato: Artigo
Idioma:Inglês
Publicado em: 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3969856/
https://ncbi.nlm.nih.gov/pubmed/24232249
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2013.153
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