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In vitro expansion and genetic modification of gastrointestinal stem cells as organoids
A key issue for sustainable culture of adult epithelial cells is enrichment for stem cell populations in tissue organoids. Gastrointestinal stem cells can be propagated using conditioned media from a supportive cell line (L-WRN). This protocol describes how to prepare conditioned media and culture s...
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| Hlavní autoři: | , |
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| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
2013
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3969856/ https://ncbi.nlm.nih.gov/pubmed/24232249 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2013.153 |
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