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In vitro expansion and genetic modification of gastrointestinal stem cells as organoids
A key issue for sustainable culture of adult epithelial cells is enrichment for stem cell populations in tissue organoids. Gastrointestinal stem cells can be propagated using conditioned media from a supportive cell line (L-WRN). This protocol describes how to prepare conditioned media and culture s...
Tallennettuna:
| Päätekijät: | , |
|---|---|
| Aineistotyyppi: | Artigo |
| Kieli: | Inglês |
| Julkaistu: |
2013
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| Aiheet: | |
| Linkit: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3969856/ https://ncbi.nlm.nih.gov/pubmed/24232249 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2013.153 |
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