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Rapid determination of homing endonuclease DNA binding specificity profile

Evaluating the binding specificity and identifying the most preferred target sequence for a homing endonuclease often represents a key step during its characterization, engineering and application for genome engineering. This chapter describes a high throughput, fluorescence based, competition bindi...

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Bibliographic Details
Main Authors: Zhao, Lei, Stoddard, Barry L.
Format: Artigo
Language:Inglês
Published: 2014
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3969613/
https://ncbi.nlm.nih.gov/pubmed/24510265
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-62703-968-0_10
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