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Effect of siRNA terminal mismatches on TRBP and Dicer binding and silencing efficacy

To enhance silencing and avoid off-target effects, siRNAs are often designed with an intentional bias to ensure that the end of the siRNA that contains the guide strand 5′ end is less stably hybridized relative to the end containing the passenger strand 5′ end. One means by which this is accomplishe...

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Autori principali: Kini, Hemant K., Walton, S. P.
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2009
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3967519/
https://ncbi.nlm.nih.gov/pubmed/19811537
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/j.1742-4658.2009.07364.x
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