Efficient Designer Nuclease-Based Homologous Recombination Enables Direct PCR Screening for Footprintless Targeted Human Pluripotent Stem Cells

Genetic engineering of human induced pluripotent stem cells (hiPSCs) via customized designer nucleases has been shown to be significantly more efficient than conventional gene targeting, but still typically depends on the introduction of additional genetic selection elements. In our study, we demons...

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Bibliographische Detailangaben
Hauptverfasser: Merkert, Sylvia, Wunderlich, Stephanie, Bednarski, Christien, Beier, Jennifer, Haase, Alexandra, Dreyer, Anne-Kathrin, Schwanke, Kristin, Meyer, Johann, Göhring, Gudrun, Cathomen, Toni, Martin, Ulrich
Format: Artigo
Sprache:Inglês
Veröffentlicht: Elsevier 2014
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Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3966116/
https://ncbi.nlm.nih.gov/pubmed/24678453
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.stemcr.2013.12.003
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