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New quantitative methods for measuring plasmid loss rates reveal unexpected stability

Plasmid loss rate measurements are standard in microbiology and key to understanding plasmid stabilization mechanisms. The conventional assays eliminate selection for plasmids at the beginning of the experiment and screen for the appearance of plasmid-free cells over long-term population growth. How...

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Autors principals: Lau, Billy T.C., Malkus, Per, Paulsson, Johan
Format: Artigo
Idioma:Inglês
Publicat: 2013
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3966108/
https://ncbi.nlm.nih.gov/pubmed/24042048
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.plasmid.2013.07.007
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