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Measuring the Rate of Conjugal Plasmid Transfer in a Bacterial Population Using Quantitative PCR

Horizontal transfer of genes between species is an important mechanism for bacterial genome evolution. In Escherichia coli, conjugation is the transfer from a donor (F(+)) to a recipient (F(−)) cell through cell-to-cell contact. We demonstrate what we believe to be a novel qPCR method for quantifyin...

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Detalhes bibliográficos
Main Authors: Wan, Zhenmao, Varshavsky, Joseph, Teegala, Sushma, McLawrence, Jamille, Goddard, Noel L.
Formato: Artigo
Idioma:Inglês
Publicado em: The Biophysical Society 2011
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3127179/
https://ncbi.nlm.nih.gov/pubmed/21723834
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2011.04.054
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