Simple and nonradioactive detection of microRNAs using digoxigenin (DIG)-labeled probes with high sensitivity

The discovery of microRNAs (miRNAs), which are ∼21–23 nucleotides that can regulate targeted mRNA by transcript cleavage or protein translation suppression, has changed the landscape of biomedical field greatly. At present, Northern blot analysis based on radioisotopes is still the most popular meth...

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Main Authors: Wu, Wei, Gong, Pengtao, Li, Jianhua, Yang, Ju, Zhang, Guocai, Li, He, Yang, Zhengtao, Zhang, Xichen
Formáid: Artigo
Teanga:Inglês
Foilsithe: Cold Spring Harbor Laboratory Press 2014
Ábhair:
Methods
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC3964919/
https://ncbi.nlm.nih.gov/pubmed/24572812
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.042150.113
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