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Simple and nonradioactive detection of microRNAs using digoxigenin (DIG)-labeled probes with high sensitivity

The discovery of microRNAs (miRNAs), which are ∼21–23 nucleotides that can regulate targeted mRNA by transcript cleavage or protein translation suppression, has changed the landscape of biomedical field greatly. At present, Northern blot analysis based on radioisotopes is still the most popular meth...

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Detalhes bibliográficos
Main Authors: Wu, Wei, Gong, Pengtao, Li, Jianhua, Yang, Ju, Zhang, Guocai, Li, He, Yang, Zhengtao, Zhang, Xichen
Formato: Artigo
Idioma:Inglês
Publicado em: Cold Spring Harbor Laboratory Press 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3964919/
https://ncbi.nlm.nih.gov/pubmed/24572812
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.042150.113
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