Nonradioactive in situ hybridization using digoxigenin-labeled oligonucleotides. Applications to musculoskeletal tissues.

We have optimized a technique for in situ localization of specific mRNAs using digoxigenin-11-dUTP-labeled oligonucleotide probes. DNA probes were synthesized for type I and type II collagen as well as transforming growth factor-beta 1 and 2 (TGF beta 1 and TGF beta 2). Control experiments, such as...

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Bibliographic Details
Main Authors: Crabb, I. D., Hughes, S. S., Hicks, D. G., Puzas, J. E., Tsao, G. J., Rosier, R. N.
Format: Artigo
Language:Inglês
Published: 1992
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC1886689/
https://ncbi.nlm.nih.gov/pubmed/1519665
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