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Controlling the enantioselectivity of enzymes by directed evolution: Practical and theoretical ramifications
A fundamentally new approach to asymmetric catalysis in organic chemistry is described based on the in vitro evolution of enantioselective enzymes. It comprises the appropriate combination of gene mutagenesis and expression coupled with an efficient high-throughput screening system for evaluating en...
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
National Academy of Sciences
2004
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| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC395973/ https://ncbi.nlm.nih.gov/pubmed/15079053 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0306866101 |
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