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Modulation of tyrT promoter activity by template supercoiling in vivo.

We have found that initiation of RNA synthesis at the tyrT promoter of Escherichia coli can be stimulated on a plasmid by a factor of 4-6 by elevation of DNA supercoiling in vivo. Increased unconstrained plasmid supercoiling was achieved by inserting the tyrT promoter upstream of the tetracycline re...

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Bibliografske podrobnosti
Main Authors: Bowater, R P, Chen, D, Lilley, D M
Format: Artigo
Jezik:Inglês
Izdano: 1994
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC395530/
https://ncbi.nlm.nih.gov/pubmed/7988562
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