Modulation of tyrT promoter activity by template supercoiling in vivo.
We have found that initiation of RNA synthesis at the tyrT promoter of Escherichia coli can be stimulated on a plasmid by a factor of 4-6 by elevation of DNA supercoiling in vivo. Increased unconstrained plasmid supercoiling was achieved by inserting the tyrT promoter upstream of the tetracycline re...
Gardado en:
| Publicado en: | EMBO J |
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| Principais autores: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
Nature Publishing Group
1994
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC395530/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7988562/ https://ncbi.nlm.nih.govhttps://doi.org/10.1002/j.1460-2075.1994.tb06903.x |
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