Modulation of tyrT promoter activity by template supercoiling in vivo.

We have found that initiation of RNA synthesis at the tyrT promoter of Escherichia coli can be stimulated on a plasmid by a factor of 4-6 by elevation of DNA supercoiling in vivo. Increased unconstrained plasmid supercoiling was achieved by inserting the tyrT promoter upstream of the tetracycline re...

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Bibliografische Detailangaben

Veröffentlicht in:	EMBO J
Hauptverfasser:	Bowater, R P, Chen, D, Lilley, D M
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	Nature Publishing Group 1994
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC395530/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7988562/ https://ncbi.nlm.nih.govhttps://doi.org/10.1002/j.1460-2075.1994.tb06903.x
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