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Niche-independent high-purity cultures of Lgr5(+) intestinal stem cells and their progeny
Although Lgr5(+) intestinal stem cells have been expanded in vitro as organoids, homogeneous culture of these cells has not been possible thus far. Here we show that two small molecules, CHIR99021 and valproic acid, synergistically maintain self-renewal of mouse Lgr5(+) intestinal stem cells, result...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3951815/ https://ncbi.nlm.nih.gov/pubmed/24292484 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.2737 |
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