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REFINING MEMBRANE PENETRATION BY A COMBINATION OF STEADY-STATE AND TIME-RESOLVED DEPTH-DEPENDENT FLUORESCENCE QUENCHING
Accurate determination of the depth of membrane penetration of a fluorescent probe, attached to lipid, protein or other macromolecule of interest, using depth-dependent quenching methodology is complicated by thermal motion in the lipid bilayer. Here we suggest that a combination of steady-state and...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3947187/ https://ncbi.nlm.nih.gov/pubmed/24141077 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2013.10.015 |
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