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REFINING MEMBRANE PENETRATION BY A COMBINATION OF STEADY-STATE AND TIME-RESOLVED DEPTH-DEPENDENT FLUORESCENCE QUENCHING

Accurate determination of the depth of membrane penetration of a fluorescent probe, attached to lipid, protein or other macromolecule of interest, using depth-dependent quenching methodology is complicated by thermal motion in the lipid bilayer. Here we suggest that a combination of steady-state and...

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Bibliografiske detaljer
Main Authors: Kyrychenko, Alexander, Ladokhin, Alexey S.
Format: Artigo
Sprog:Inglês
Udgivet: 2013
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3947187/
https://ncbi.nlm.nih.gov/pubmed/24141077
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2013.10.015
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