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Functional assay for T4 lysozyme-engineered G Protein-Coupled Receptors with an ion channel reporter
SUMMARY: Structural studies of G protein-coupled receptors (GPCRs) extensively use the insertion of globular soluble protein domains in order to facilitate their crystallization. However, when inserted in the third intracellular loop (i3 loop), the soluble protein domain disrupts their coupling to G...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3947161/ https://ncbi.nlm.nih.gov/pubmed/24268646 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.str.2013.10.002 |
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