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C-Terminal Protein Characterization by Mass Spectrometry: Isolation of C-Terminal Fragments from Cyanogen Bromide-Cleaved Protein

A sample preparation method for protein C-terminal peptide isolation from cyanogen bromide (CNBr) digests has been developed. In this strategy, the analyte was reduced and carboxyamidomethylated, followed by CNBr cleavage in a one-pot reaction scheme. The digest was then adsorbed on ZipTip(C18) pipe...

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Autori principali: Nika, Heinz, Hawke, David H., Angeletti, Ruth Hogue
Natura: Artigo
Lingua:Inglês
Pubblicazione: Association of Biomolecular Resource Facilities 2014
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3942263/
https://ncbi.nlm.nih.gov/pubmed/24688319
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7171/jbt.14-2501-001
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