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N-Terminal Protein Characterization by Mass Spectrometry after Cyanogen Bromide Cleavage using Combined Microscale Liquid- and Solid-Phase Derivatization

A sample preparation method for protein N-terminal peptide isolation from cyanogen bromide (CNBr) protein digests has been developed. In this strategy, the CNBr cleavage was preceded by protein α- and ε-amine acetylation and carboxyamidomethylation in a one-pot reaction scheme. The peptide mixture w...

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Detalhes bibliográficos
Main Authors: Nika, Heinz, Hawke, David H., Angeletti, Ruth Hogue
Formato: Artigo
Idioma:Inglês
Publicado em: Association of Biomolecular Resource Facilities 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3942260/
https://ncbi.nlm.nih.gov/pubmed/24688320
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7171/jbt.14-2501-003
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